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Successfully introduce transgenes using the Safe Harbor system

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It can be difficult to successfully introduce a transgene into a different genome. Often times, the gene is not expressed properly or it may silence another gene, possibly causing a synthetic lethality. With the use of the Safe Harbor system, such issues will be circumvented as the transgene will be integrated at a known locus that expresses the gene reliably and without disruption of another coding gene. The Safe Harbor site was originally discovered in mouse at the Rosa26 locus; and in 2010, a similar locus was discovered in the human: the AAVS1 locus.  

Applications for Safe Harbor technology include:

  • ♦ Rescue your knockout by placing the GOI at the Safe Harbor site.
  • ♦ Explore allotropic expression: achieve nuclear expression of mitochondrial genes.
  • ♦ Study xenotropic expression: express plant genes in a mammalian system.
  • ♦ Combat against genetic instability, dosage effects, and epigenetic consequences.
  • ♦ Accomplish robust gene correction and successful transgene integration.
  • ♦ Survey gene delivery in autologous cell therapy.

The application note for the use of Safe Harbor technology for transgene integration in mice and human cell lines available at the link below, summarizes our knowledge of the Safe Harbor sites in each genome, their discovery, applications for your own research and how GeneCopoeia’s products can boost your project.

GeneCopoeia, Inc.
Download the Safe Harbor Application Note