Thermo Scientific Launches HeLa Cell-Based IVT System
Product News Apr 19, 2012
Thermo Fisher Scientific has introduced the Thermo Scientific 1-Step Human High Yield In Vitro Translation (IVT) Kits.
The new kits have a continuous-feed device and can produce 10- to 100-times more functional protein per milliliter than other eukaryotic IVT systems.
The protein obtained with the 1-Step Human High Yield IVT Kit can be used for structural analysis, antibody production and kinetic or enzymatic assays.
The kit uses modified HeLa cell extracts to provide the protein expression machinery.
Protein expression is performed using a proprietary mini-dialysis device that allows the continuous supply of nucleotides, amino acids and energy-generating substrates to extend the protein translation reaction.
The device also allows the removal of protein synthesis inhibitors. With the kit, protein expression can continue for up to 24 hours, providing protein yields ranging from 250 to 750 micrograms per milliliter.
The complete 1-Step High Yield IVT Kits supply all components necessary for the transcription and translation of protein, including an optimized expression vector, pT7CFE1-CGST-HA-His.
This triple-tagged vector (C-terminal GST, HA and His6) contains numerous features that are critical for high-level translation.
For more information on the 1-Step Human High Yield IVT Kit, please visit www.thermoscientific.com/pierce.