Tissue Dissociation Guide
Worthington's Tissue Dissociation Guide assists researchers with selecting enzymes, developing and optimizing protocols and better understanding the process of enzymatic primary cell isolation and tissue dissociation.
Researchers searching the scientific literature for information on the ideal enzymes and optimal conditions for tissue dissociation are often confronted with conflicting data. Much of the variation stems from the complex and dynamic nature of the extracellular matrix and from the historical use of relatively crude, undefined enzyme preparations for cell isolation applications. Also, the extracellular matrix is composed of a wide variety of proteins, glycoproteins, lipids and glycolipids, all of which can differ in abundance from species to species, tissue to tissue and with developmental age. Commonly used crude enzyme preparations such as Pronase, NF 1:250 and collagenase contain several proteases in variable concentrations, as well as a variety of polysaccharidases, nucleases and lipases.
This Tissue Dissociation Guide summarizes our knowledge of how these enzymes accomplish the "routine" operations of tissue dissociation and cell harvesting, describes standard lab procedures, offers a logical experimental approach for establishing a cell isolation protocol, and lists many tissue specific references.
Worthington Biochemical Corporation
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