ZYGEM Releases Application Note Showing Its DNA Extraction Kits Produce High Yields of Quality DNA
Product News May 08, 2009
ZyGEM Corp. Ltd. has announced the availability of a new application note documenting the performance advantages of its prepGEM™ kits compared to other commercial kits for small animal genotyping. The note specifically evaluates ZyGEM’s prepGEM™ Tissue DNA extraction kits used in PCR assays for genotyping mice, a commonly-performed biomedical research application.
In the application note, ZyGEM researchers describe their experience extracting DNA and determining precise genotypes from mouse tail samples. Researchers performed extractions comparing ZyGEM’s prepGEM™ Tissue kit with two other commercially available kits with similar protocols. They then amplified a relevant segment of DNA using PCR reagents supplied in the kits and also with an external amplification system. The prepGEM™ Tissue extractions and amplifications yielded superior results based on both qualitative and quantitative PCR comparisons of the amplified DNA.
“This new application note focusing on mouse genotyping demonstrates that our prepGEM™ Tissue kits easily produce rapid, robust and reliable DNA extraction that outperformed the other kits tested,” said Paul Kinnon, CEO of ZyGEM. “Since small animal genotyping is a critical but often time-consuming aspect of biomedical research, we are pleased to make this comparative data available to our growing customer base.”
The results included in this application note show that in a PCR assay gel analysis, the prepGEM™ Tissue kit extractions yielded readily amplified DNA with no migration distortion or smearing, while the other two commercial kits yielded DNA with migration distortion or low band intensity, indicating a low yield of extracted DNA.
ZyGEM claims that the quantitative PCR analysis showed that samples prepared with prepGEM™ Tissue yielded over 34 times the amount of DNA produced by the higher-yielding of the two comparison kits.
The prepGEM™ Tissue kit is compatible with all PCR detection and amplification reagents, and the stability of the prepGEM™ reagents allows storage at 4°C, reducing preparation time.