bYlok® Bispecific Pairing Technology: A Close to Nature Solution to Overcome Chain Mispairing
Lonza’s bYlok® technology is changing the way bispecific antibodies are designed. Achieving the correct heterodimerization of light and heavy chains during bispecific production can be challenging, and whilst the knobs-into-holes technology solves heavy chain mispairing, bYlok® offers an easy to implement solution to address heavy chain-light chain (HC-LC) mispairing. Bispecific antibodies designed with bYlok® technology achieve >95%* correct HC-LC pairing efficiency.
The bYlok® technology works on the principle of being closer-to-nature by design, reducing the risk of any unwanted immunogenicity, whilst offering superior manufacturing efficiencies to ease bispecific production at scale. It involves the engineering of the native disulphide bridge, moving it from the constant domain (CH1/CL) to the variable domain (VH/VL). This minor modification favours the formation of correct HC-LC heterodimers.
Benefits of bYlok®:
1. Simple engineering that can be retrofitted
The platform technology can be used to generate IgG-like bispecific complexes and other types of complexes from IgGs already being used in the clinic. By combining bispecific pairing precision with no further impact on predicted immunogenicity*, bYlok® technology can help you to develop better products.
2. No compromises to the bispecific design
The bYlok® mutation is a minor modification to the original mAb design, enabling a closer-to-nature approach to reduce the risk of unwanted immunogenicity
3. Standard product purification methods
Unlike other technologies, you don’t have to sacrifice timelines or yields to purify the correct bispecific format. With bYlok® technology, you can use standard purification methods.
4. An easily implemented solution for everybody to use
Try bYlok® for yourself with our Lonza in Your Lab® license offering. You’ll get access to our proprietary bYlok® Sequence Editor - our online tool that guides you on how to introduce the required sequence modification and you’ll be supported by our network of dedicated experts.
*Lonza internal data
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