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ioGABAergic Neurons – iPSC-Derived Inhibitory Neurons With >95% Purity

ioGABAergic Neurons – iPSC-Derived Inhibitory Neurons With >95% Purity

ioGABAergic Neurons are human induced pluripotent stem cell (iPSC)-derived inhibitory GABAergic neurons, precision reprogrammed using opti-ox™technology, that express classical marker genes including GAD1, GAD2, VGAT, DLX1 and DLX2. These cells provide an easy to use, high-quality, co-culture compatible human model for the study of neurological circuitry, neurodevelopmental (autism, epilepsy) and neurodegenerative (Alzheimer’s) disease.

Using a simple 2-step protocol, consistently go from cryopreserved cells to mature, functional, experiment-ready GABAergic neurons with over 95% purity in just 12 days, as confirmed by single cell RNA sequencing. The high purity of ioGABAergic Neurons solves the problem of heterogeneous neuronal populations, obtained with traditional directed differentiation protocols, where excitatory neurons often obscure inhibitory signals in cell-based assays.

Obtain reproducible results from your assays, regardless of the lot, assay, user, or lab, and save valuable time on batch validation. No prior experience of culturing iPSC-derived cells is required as every vial of ioGABAergic Neurons will consistently generate a highly defined cell population, as confirmed by bulk RNA sequencing.

Calcium imaging has been used to demonstrate that ioGABAergic Neurons display spontaneous activity.  In the human brain, the functions of GABAergic neurons are intimately connected with glutamatergic neurons and astrocytes, making co- or tri-culturing these cell types desirable in vitro. ioGABAergic Neurons are suitable for co- and tri-culture studies with astrocytes and glutamatergic neurons, to more closely mirror and study in vivo brain function.

Fill out the form to speak to an expert about ioGABAergic Neurons. 

Product Specifications
Starting material Human iPSC line
Donor Caucasian adult male (skin fibroblast)
Karyotype Normal (46, XY)
Differentiation method opti-ox cellular reprogramming
Format Cryopreserved cells
Quality control Sterility, protein expression (ICC) and gene expression (RT-qPCR)
Vial size >3 x 10⁶ viable cells
Seeding compatibility 6, 12, 24 & 96 well plates
Recommended seeding density 150,000 cells/cm²
Product use Research use only
About bit.bio
bit.bio is an award-winning human synthetic biology company whose mission is to code cells for novel cures. They have developed an end-to-end platform for the creation of any human cell type. With their cutting-edge and patent-protected opti-ox<sup>™</sup> precision cell programing technology, bit.bio can deterministically program human induced pluripotent stem cells (iPSCs) into a chosen cell identity with unprecedented biological consistency at an industrial scale and approximately 10 times faster than conventional methods. Their platform has the potential to unlock a new generation of medicines.
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