Single-Cell Westerns from ProteinSimple

Meet Milo, the world’s first Single-Cell Western platform.  Milo brings the traditional Western blot to the world of single-cell analysis for the first time.  Milo makes Western-based protein expression measurements on ~1,000 single-cells in parallel. Quantify protein expression heterogeneity in your sample by quantifying target abundance in each single cell.  Use standard Western antibodies to multiplex up to 12 proteins per cell.  Now you can validate your single-cell RNA-seq data at the protein level to confirm your gene expression hypotheses are true at the functional protein level. Milo can quantify CRISPR editing efficiency or transfection efficiency.  Milo can also simplify your flow experiments by detecting surface, intracellular and even intranuclear proteins with the same simple workflow. Stop hunting for good flow cytometry or mass cytometry antibodies. Milo provides protein size and immune binding information which enables highly specific protein expression measurements at the single-cell level.  Milo can even measure protein isoform heterogeneity by resolving protein isoforms by size and then quantifying abundance of each specific isoform in each cell.  Use Milo to unlock the single-cell proteome in your lab!