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Maximizing CRISPR Success With High-Purity gRNA

Video  
Published: October 2, 2025 
Laura Hemmingham, PhD
 speaking with 
Cody Palumbo, PhD

CRISPR is transforming biopharmaceutical research, but the reliability of genome edits depends heavily on the quality of guide RNAs (gRNAs). Conventional desalting purification methods often leave behind unwanted byproducts that compromise results.

High-purity gRNAs are essential for accurate, reproducible editing. By leveraging high-performance liquid chromatography (HPLC) purification, researchers can eliminate synthesis impurities, reduce variability and improve fidelity – accelerating timelines and increasing confidence in downstream studies.

In this Teach Me in 10, Dr. Cody Palumbo, a Senior Application Scientist in Technical Design at TriLink BioTechnologies, discusses why gRNA purity matters and how advanced purification solutions can streamline development.


Watch this episode to learn:
  • Why HPLC purification outperforms desalting in generating high-purity gRNAs
  • How low-purity gRNAs impact editing efficiency and reproducibility in development programs
  • Best practices for ensuring gRNA purity, from synthesis through scalable cleanup and quality control

Further resources:
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Meet the Author
A picture of Laura Hemmingham, PhD
Laura Hemmingham, PhD
Scientific Content Producer
Laura holds a BSc in Zoology from Queen Mary University of London, an MSc in Palaeoanthropology from UCL, and a PhD in Environmental Sciences from Royal Holloway, University of London. Her academic journey has been fueled by a broad interest in the life sciences and a passion for making complex science accessible to diverse audiences. Before joining Technology Networks in 2025, she gained experience across several areas of science communication, including e-learning, external communications, and science writing. Her work has focused on translating technical research into engaging, understandable content tailored for both specialist and general audiences. As a Scientific Content Producer for custom video content at Technology Networks, Laura is particularly interested in bridging the gap between scientific discovery and public engagement through creative storytelling. Her work aims to support scientific literacy while highlighting the real-world impact of research and innovation.
Interviewing
A picture of Cody Palumbo, PhD
Cody Palumbo, PhD
Senior Application Scientist
Dr. Cody Palumbo is a Senior Application Scientist in Technical Design at TriLink BioTechnologies, where he partners with customers to support gRNA, oligonucleotide and gene editing workflows by providing technical guidance and customized solutions. He brings deep expertise in CRISPR-based technologies and therapeutic development, drawing on years of experience across biotech and pharmaceutical settings. Prior to TriLink, Dr. Palumbo served as Associate Director of Gene Editing Process Development and Tech Transfer at Sana Biotechnology and led a team of Process Development scientists at Bristol Myers Squibb focused on gene editing tools for cell therapies. He holds a Ph.D. in Chemistry from the University of California, Davis. Dr. Palumbo is passionate about bridging technical innovation with real-world impact and frequently speaks on the evolving landscape of genome editing technologies.
Related Topic Pages
Liquid Chromatography
Gene and Cell Therapy
CRISPR and Genome Editing
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