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Preventing Ex Vivo Activational Confounds in Single-Cell Neuroscience Studies

A long-standing concern for neuroscientists studying specific cell types in vivo is whether cell isolation from CNS tissue induces ex vivo activational artifacts that confound experimental interpretations. Here we present best practices for isolating glial and neuronal cells for downstream omics analyses. We show that the inclusion of transcription and translation inhibitors during cell preparation minimizes ex vivo activation induced during enzymatic and mechanical dissociation. We will also compare cell preparation and isolation techniques for various CNS cell types.


Attend this webinar to:

  • Learn about two techniques that can be used to prevent ex vivo activational confounds in neuroscience studies
  • Determine the best cell preparation and isolation technique for isolating different CNS cell types
  • Discover why it is important to consider ex vivo activational confounds when designing neuroscience studies
Speakers
Dr. Sarah Ocañas
Dr. Sarah Ocañas
Assistant Member of the Genes and Human Disease Research Program, Oklahoma Medical Research Foundation
Dr. Bill Freeman
Dr. Bill Freeman
Member of the Genes and Human Disease Research Program, Oklahoma City Veterans Affairs Medical Center
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