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Enhanced Mouse Brain Cell Surface Expression – A Comparison to Enzymatic Methods

Neuro mapping

As researchers endeavor to map the architecture of the brain, many rely on tissue dissociation approaches to prepare single-cell suspensions for downstream analysis. However, such conventional methods can involve enzymes and heat, which are known to induce transcriptional and proteotype changes in brain cells.

Choosing a more gentle approach can help researchers to effectively characterize brain cell populations while protecting the characteristics of precious tissue samples.  

This whitepaper highlights a tissue dissociation technology that uses acoustic energy to generate single-cell suspensions that maintain the characteristics of important cellular subsets. 

Download this whitepaper to discover:

  • The importance of preserving cell surface characteristics in brain tissue samples
  • An enzyme- and heat-free approach that can generate more individual cells than conventional methods
  • How the latest technology can help researchers identify rare cell subpopulations
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