Paired Blind Polytrode and Juxtacellular Recordings In Vivo

Paired electrophysiological recordings using more than one electrode in vivo can be challenging. Visual guidance of pipettes can be achieved by combining 2-Photon imaging with the technique. However, this approach is expensive, limited to depths of 1mm and requires that the electrodes fluoresce. In this white paper the authors describe a more cost effective solution. Their approach is to combine computer controllable motorised micromanipulators with trigonometric calculations of the electrode positions in the tissue.

In this white paper, the authors describe a straightforward algorithm  and method to achieve blind ‘paired recordings’ in vivo in a reliable manner, using Scientifica micromanipulators.