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Application Notes

 
Achieve Greater Reliability in Western Blot Loading Controls
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Download this app note to learn more about band signals and how they can be used in western blots.

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Targeted Proteomics: Achieving High Throughput and High Sensitivity
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Download this app note to learn more about proteomic targets, including associated technologies and how to achieve high reproducibility.

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How To Increase Recovery at Critical Protein Samples: Impact of Syringe Filter Membrane, Volume and pH
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Download this app note to learn more about the determination of membranes used for protein analysis.

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Achieve Successful Immunoprecipitation With Dynabeads
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Immunoprecipitation in its basic form is very simple, however protein–protein binding often involves transient and weak interactions. It is therefore paramount to use a method that offers rapid binding kinetics and low nonspecific binding.

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Proteomic Interrogation of Primary Insulin-Secreting Pancreatic β-cells
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Dr Jarrod Marto’s lab from the Dana-Farber Cancer Institute have developed a new approach to identify expression of low-level enzymes for the first time in primary insulinsecreting beta cells (β-cells). In their research, they found that these proteins are critical, functional components of key metabolic pathways which can support β-cell survival under inflammatory stress.

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Vitamin D Biomarker Quantitation in Plasma: Improving Limits of Detection
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Vitamin D is widely monitored and used as an indicator for skeletal health in both children and adults with deficiencies resulting in rickets and osteoporosis. This app note details a simple extraction of OH-Vit D2 and OH-Vit D3 from human plasma.

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Simple Steps To Publication-Quality Western Blots
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Download this app note to discover tips on ensuring the reproducibility and quality of your western blotting data.

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Safety, Sample Integrity and Throughput With Semi-Automated Sample Tube Decapping
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Download this app note to discover how semi-automated sample tube decapping can ensure sample integrity whilst also helping to prevent injury.

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Handbook of ICP-QQQ Applications Using the Agilent 8800 and 8900
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In this application compendium, discover the true breadth of ICP-QQQ and how it can be applied for trace element analysis at low concentration, high reliability and high confidence.

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µPAC™ capLC Columns: The Promise of Repeatability, Robustness, Throughput and Sensitivity for Proteomics Research
Application Note

Download this article to learn more about nanoLC-MS and capillaryLC-MS and the benefits of μPAC™ for proteomics research.

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