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Application Notes

 
Simultaneous Detection of Copy Number and Copy-Neutral LOH Using a Single Microarray
Application Note

Agilent Technologies

Application note describing the Agilent SurePrint G3 CGH+SNP microarray platform for the simultaneous detection of copy number and copy-neutral genetic aberrations.

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BD Cytometric Bead Array Cell Signaling Flex Set System
Application Note

BD Biosciences

The BD™ Cytometric Bead Array (CBA) Cell Signaling Flex Set system lets you achieve high productivity in cell signaling studies by extracting more data from less sample faster than traditional Western blot techniques.

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Promega’s ADP-Glo™ Kinase Assay performed on BMG LABTECH’s PHERAstar FS and POLARstar Omega
Application Note

BMG Labtech

The PHERAstar FS as well as the POLARstar Omega offer the ability to perform the ADP-Glo™ assay from Promega in a 384-well format. The ADP-Glo™ Kinase Assay is a homogenous luminescent assay to detect ADP in concentrations ranging from 1 mM to 0.01 µM. Instrument settings and standard ATP/ADP curves over a wide concentration range are presented for both the PHERAstar FS and the POLARstar Omega.

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RADAR, a Xevo TQ-S function, combined with Low-High pH UPLC Screening for Fast, Simple Bioanalysis Methods Development
Application Note

Washington University School of Medicine

Here, we demonstrate the productivity gains possible with Xevo TQ-S for use in bioanalytical methods. Xevo TQ-S employs a unique RADAR functionality to collect data in the time scale of a narrow 1- to 2-second peak.

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Monitoring Bacterial Cell-to-Cell Communication “Quorum sensing” Using FLUOstar OPTIMA
Application Note

BMG Labtech

Bacteria can monitor and respond to changes to environmental conditions via a cell density process known as quorum sensing. Bacteria use this process to monitor their community by producing, detecting and responding to low molecular mass signal molecules, called autoinducers. Effects on quorum sensing were investigated by measuring growth and bioluminescence of Vibrio fischeri. The FLUOstar OPTIMA was utilized to measure absorbance and luminescence in parallel.

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alamarBlue® Assay for Assessment of Cell Proliferation using the FLUOstar OPTIMA
Application Note

BMG Labtech

The alamarBlue® cell proliferation assay is based on a reducing environment that indicates metabolic active cells. Active cells are able to convert resazurin into the resorufin. Resazurin is blue and non-fluorescent whereas resorufin is red and highly fluorescent; therefore a fluorescent and a colorimetric detection are possible. This application notes focuses on the quantification of cell proliferation in a Human Osteoblast cell culture by measuring alamarBlue® fluorescence on the FLUOstar OPTIMA microplate reader.

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A DELFIA® time-resolved fluorescence cell-mediated cytotoxicity assay performed on the PHERAstar
Application Note

BMG Labtech

A europium based time-resolved fluorescence cytotoxicity assay was used to study the loss of membrane integrity of target cells. For that cells are loaded with a fluorescence enhancing ligand that penetrates the cell membrane quickly and is hydrolized to form a hydrophilic ligand that is released from cells only after cytolysis. The data obtained with BMG LABTECH´s PHERAstar shows that estrogen dramatically reduces cell induced by natural killer cells.

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Detection of NADH and NADPH with the Omega’s High Speed, Full UV/Vis Absorbance Spectrometer
Application Note

BMG Labtech

NAD+, NADH as well as NADP+, NADPH have been known to play vital roles in energy metabolism, antioxidation, and reductive biosynthesis. Changes in these systems can be monitored using absorbance spectroscopy. NADH and NADPH absorb light at 340 nm, whereas NAD+ and NADP+ do not. The FLUOstar, POLARstar and SPECTROstar Omega microplate readers from BMG LABTECH all have a UV/Vis spectrometer that can measure any absorbance range from 220-850 nm.

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