Automating ELISAs on Tecan’s Freedom EVO® using Optimiser™ technology from Siloam Biosciences
ELISAs are considered one of the most useful secondary or tertiary type assays in drug discovery, because they elucidate specific cellular pathways and associated mechanisms of action for target genes, proteins or small molecules. They are equally important to clinical biology laboratories, as they enable determination of biomarker concentrations in unknown biological samples.
There have been a number of attempts to replace the traditional plate-based ELISA with microfluidic-based technology, but in general these have all suffered from the need for specialized liquid handling systems. Until now, microfluidic technology has not been adapted to the SBS plate footprint, and could not make use of the plate-based liquid handling and detection instrumentation found in many life science laboratories.