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Development of a Targeted Plasma Quantitation Assay Using dia-PASEF

In clinical proteomics research applications, blood plasma is the key sample of interest, but comprehensive proteome analysis can be challenging due to the large dynamic range of concentration.

The ideal method to perform large-cohort targeted proteomics analysis on plasma samples would include fast single-shot analysis without the need for tedious optimization for each targeted peptide.

Download this app note to learn how dia-PASEF was used to create an assay that:

  • Yields excellent targeted quantitation results
  • Eliminates tedious and time-consuming method development
  • Can measure and quantify proteins outside the target pane