Phosphorylated Peptide Post-Translational Modification Site Localization and Isomer Differentiation
Protein phosphorylation is an important post-translational modification (PTM) as it is involved in a large variety of dynamic cellular processes. However, PTM site localization and quantification of phosphopeptides by collision induced dissociation MS/MS can be challenging, and phospho-peptides can exhibit a partial neutral loss of the phospho group.
Download this app note to learn how electron activated dissociation fragmentation:
- Shows promising performances for the robust and accurate quantification of labile PTMs
- Generates strong and distinct PTM site localization ions
- Offers a detailed characterization of the modified peptides of interest