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Sensitive Quantification and Structural Elucidation of Steroids

credit: iStock

Hormonal steroids regulate the majority of bodily functions, and the dysregulation of these molecules can play a role in the pathophysiology of human disease. 

Early techniques to measure endogenous steroids, such as immunoassays, are problematic because they lack specificity for low-level steroids. Gas chromatography-mass spectrometry offers higher specificity and is sensitive to low-level steroids but requires extensive sample preparation.

This app note highlights why liquid chromatography-tandem mass spectrometry (LC-MS/MS), paired with electron-activated dissociation (EAD)-based fragmentation, has emerged as a key technique for steroid analysis.

Download this app note to discover:

  • A method that enables robust, high-throughput analysis of hormonal steroids in human plasma
  • Fragment ion generation that allows characterization not achievable using collision-induced dissociation
  • How to improve analyte specificity during quantitative analysis
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