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Shotgun Proteomics for Unbiased Quantification

Shotgun Proteomics for Unbiased Quantification content piece image

Shotgun proteomics has the capacity to deliver in-depth information on the identification, the relative amount, and the modification patterns of ever larger fraction of the proteins present in a sample. This has made it an essential approach in most biological research areas. The recent introduction of Parallel Accumulation and SErial Fragmentation (PASEF) technology and the corresponding development of 4D-Proteomics™ approaches has really boosted the potential of these techniques by eliminating the usual need to compromise selectivity or sensitivity for throughput and robustness, by simultaneously improving all these parameters.

Download this app note to discover how timsTOF Pro 2 can:

  • Measure over 7000 proteins in a 70 min LC separation
  • Deliver high identification and quantification rates
  • Offer increased speed and depth of coverage