Tryptic Mapping Automated on the CyBi®-RoboSpense and Integration of an Agilent 1100/1200 HPLC System for Peptide Analysis

Peptide mapping is an important analytical technique widely used to study the primary structure of proteins. In quality control settings, peptide mapping is employed as an identity test to probe for small changes in protein primary structure. Long incubation times and time consuming purification steps characterize this method. The complete manual preparation process takes about eight hours occupying one technician a whole day. Therefore, automation of the process is desired. An additional benefit of automation is data continuity and reproducibility.