Drift time-specific collision energies enable deep-coverage data-independent acquisition proteomics
Article May 11, 2014
Ute Distler, Jörg Kuharev, Pedro Navarro, Yishai Levin, Hansjörg Schild, Stefan Tenzer
Abstract: We present a data-independent acquisition mass spectrometry method, ultradefinition (UD) MSE. This approach utilizes ion mobility drift time-specific collision-energy profiles to enhance precursor fragmentation efficiency over current MSE and high-definition (HD) MSE data-independent acquisition techniques. UDMSE provided high reproducibility and substantially improved proteome coverage of the HeLa cell proteome compared to previous implementations of MSE, and it also outperformed a state-of-the-art data-dependent acquisition workflow. Additionally, we report a software tool, ISOQuant, for processing label-free quantitative UDMSE data.
To pick apart the differences between individual cells in complex multicellular organisms, we need to look at cells one-by-one. This article takes a look at how several scientists in North America are using single cell proteomics (SCP) technologies to discern disease pathogenesis and enhance directed stem-cell differentiation.READ MORE
As many people spent the summer trying to keep the flies away from their fruit-bowls, an international group of scientists published one of the biggest and most important datasets in the field of connectomics to date, the complete 3D electron micrograph volume of the fruit-fly (Drosophila melanogaster) brain.READ MORE