7 Tips to Improve Your Western Blot
How To Guide Jun 22, 2018 | by Karishma Joshi, 2nd Year PhD Student, Department of Neuroscience, Psychology & Behaviour, The University of Leicester, UK
How hard can it be to get a nice single band on a clean blot? The answer, VERY!
Follow these 7 top tips to improve your westerns and reap the rewards of clean bands and publishable quality data, every time.
This how-to guide gives you practical advice on:
- Cleaning your equipment
- Making your best gel
- Setting up electrodes
- Transferring proteins from gel to membrane
- Choosing the right antibody
- Testing your antibodies
RELATED HOW TO GUIDES
The CRISPR interference (CRISPRi) machinery is an efficient, reversible and specific genome regulating platform for transcription control, without altering the target DNA sequence. This guide includes key steps on implementing CRISPRi using dCas9 derived from Streptococcus pyogenes.READ MORE
Like what you just read? You can find similar content on the communities below.Analysis & Separations Applied Sciences Biopharma Cancer Research Cell Science Diagnostics Drug Discovery Genomics Research Informatics Neuroscience Immunology & Microbiology
To personalize the content you see on Technology Networks homepage, Log In or Subscribe for FreeLOGIN SUBSCRIBE FOR FREE