How to Make Neurons: Differentiating pluripotent stem cells to cortical or hypothalamic neurons
How To Guide Oct 01, 2018 | by Peter Kirwan PhD, Postdoctoral Research Associate, Merkle Lab, University of Cambridge.
To date, most of our understanding of human biology has been extrapolated from rodents. While these systems are a valuable resource for understanding in vivo biology, there are key differences between humans and rodents, and models that enable us to understand human-specific aspects of molecular and cellular biology as well as disease have been lacking.
Over the last decade, researchers have developed methods using human pluripotent stem cells (hPSCs) to generate specific cells types, providing new model systems for probing human biology and disease, as well as delivering novel drug screening platforms.
The aim of this guide is to outline protocols for the differentiation of hPSCs to either cortical neurons or hypothalamic neurons, and to provide key pointers for researchers who are undertaking these protocols for the first time.