Top Tips for RNA Extraction From Mouse Brain Tissue
How To Guide Dec 07, 2018 | by Nora Badawy, PhD
Ribonucleic Acid (RNA) is essential for genetic regulation and expression. In many neuroscience studies, animal models such as mice are used, and accordingly, you may find that you must extract RNA from mouse brain tissue at some point or other.
There are unique challenges that arise when trying to extract RNA from mouse brain samples. This guide outlines the main precautions you should take to overcome these challenges and achieve successful RNA extraction.
Read this guide to discover:
- How to prepare your lab for safe, RNase-proof extraction
- How to collect, handle and store samples to give the best chance of successful extraction
- How to overcome key challenges in RNA extraction from mouse brain tissue
RELATED HOW TO GUIDES
Immunohistochemistry (IHC) is a technique that capitalizes on the specificity of the immune system to examine tissue stained to demonstrate the presence of certain proteins. In this guide, we have provided a sample IHC protocol followed by tips and techniques to optimize your IHC staining.