Proteins that Stabilize Sight Circuitry Found
Little is known about how axons in the developing visual system stabilize their connections upon reaching the correct layer, but scientists at Tokyo Institute of Technology (Tokyo Tech), led by Associate Professor Takashi Suzuki, have identified two proteins that provide layer-specific stabilization.
Scientists at Tokyo Tech have made an important discovery concerning the development of layer-specific axonal connections in the developing visual system of Drosophila flies. This discovery provides valuable insights into how neurons in the developing brain are wired. The molecular mechanisms we found can serve as a universal principle for wiring axons also in higher animals. We assume our findings help stabilize the regenerating axons to the desired depth in the brain layers, in the case such as neuronal transplantation after injury.
Drosophila flies, which are a common model organism in laboratory research, have a layered visual system, just like other animals, including humans. For the visual system to function properly, each layer must receive specific connections from particular neuron populations. For this to happen, axons in the developing nervous system must grow to their target layers and then form stable connections there.
To identify how these stable connections form, Takashi Suzuki's group investigated the roles of two proteins, called LAR and Ptp69D, that are necessary for a group of light-sensitive cells called R7 photoreceptors to correctly project axons to the sixth layer (M6) of a visual area called the medulla in Drosophila brains. Their work is published in eLife.
The group first created double-mutant embryonic flies in which the R7 photoreceptors did not express the LAR and Ptp69D genes. In these flies, over 80% of R7 axons failed to terminate within the M6 layer. The axons would initially reach the M6 layer, but as development progressed, they frequently retracted and exited the medulla entirely.
Next, they used a temperature-controlled gene expression system to determine how cumulative LAR and Ptp69D expression affected layer-specific termination of the R7 axons. At the lowest cumulative expression level, almost all R7 axons terminated outside the medulla, but as cumulative expression levels were increased towards normal physiological levels, it became common for R7 axons to terminate in the M6 layer.
They continued their research by selectively reintroducing LAR or Ptp69D in the double-mutant flies. Reintroducing either protein restored R7 axon termination in the medulla, but this rescue effect was inhibited when the scientists used mutations to delete parts of the restored proteins that affect functions within the neurons. This suggests that intracellular signaling from LAR and Ptp69D plays a key role in forming stable connections in the medulla.
Figure 2. LAR and Ptp69D are required for axonal stabilization
To wire the neuronal connection, the axons are first attracted then stabilize. LAR and Ptp69D are required only for the stabilization.
Interestingly, restoring LAR resulted in R7 axons mainly terminating in layers M0 and M6, whereas restoring Ptp69D resulted in R7 axons terminating in layer M3. Proteins like LAR and Ptp69D bind to target molecules called ligands, and this observation suggests that layers M0 and M6 contain ligands for LAR and that layer M3 contains ligands for Ptp69D. The presence of appropriate ligands would cause LAR and Ptp69D to form stable connections for the R7 axons.
Figure 3. Cumulative LAR and Ptp69D expression controls layer termination of R7 axons.
The final stabilizing layer of the R7 photoreceptor axons are determined by the cumulative activity of LAR and Ptp69D.
Taken together, these results provide important insights into how axon projections are targeted in the developing visual system. The effects of LAR and Ptp69D were both additive, in that cumulative expression determined the accuracy of axon targeting to the medulla, and protein-specific, in that LAR and Ptp69D tended to guide the axons to different layers in the medulla. A major task for future studies will be to identify the LAR and Ptp69D ligands that are hypothesized to mediate this layer-specific targeting.
This article has been republished from materials provided by the Tokyo Institute of Technology. Note: material may have been edited for length and content. For further information, please contact the cited source.
Hakeda-Suzuki, S., Takechi, H., Kawamura, H., & Suzuki, T. (2017). Two receptor tyrosine phosphatases dictate the depth of axonal stabilizing layer in the visual system. ELife, 6. doi:10.7554/elife.31812
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