Depressive Symptoms Related to Biological Markers of Immune Functioning among Young People with HIV
Youth living with HIV (YLWH) are at risk for depression. This study aimed to identify trends in depressive symptoms for YLWH in a specialty-care clinic and follow up clinical treatment procedures.
An Emerging Phenotype of Partial RAG 1/2 Deficiency Among Young Children with Autoimmunity and Viral Infections
We describe the natural history of a cohort of 12 patients with confirmed partial RAG1/2 mutations and autoimmunity at a young age. We were seeking the link between viral infections and autoimmunity and tested candidate biomarkers that may reflect the underlying RAG1/2 protein deficiency.
Development and Assessment of Non-Infected and Infected Skin Models Using MALDI-MSI
Infections have detrimental effects on wound healing leading to chronic wound formation. The use of labskin,a living skin model, in combination with mass spectrometry imaging, provides a good model for assessing wound healing factors.
Exploiting Polypharmacology in Precision Oncology: Identification of Differential Kinase Off-targets Among Clinical PARP Inhibitors
Can we use computational methods to identify previously unknown off-targets of PARP inhibitors that can explain their observed differences?
LC-SWATH/MS Metabolomics Platform with Hyphenation of Extraction for the Analysis of Polar and Non-polar Metabolites in Biological Samples
Automated robotic sample preparation with hyphenated dual LC-SWATH/MS analysis for lipids and polar metabolites.
Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s Disease
Here we evaluate the ability to detect changes in phosphorylation levels of ERK and CREB following treatment with Aβ using the SH-SY5Y neuroblastoma cell line.
Application of Solid Phase Micro Extraction (SPME) and On‐Fiber Derivatization to Detect Precursors of Biomolecules in the study of Proto‐metabolism
In the studies of origins of life, plausible synthetic reaction pathways to form biological relevant molecules, or building blocks of proto‐bilayer lipids, protein, RNA, and DNA in the primitive environment have been investigated intensively.
Assessment of Oral LISPRO Treatment in Ameliorating Amyloid and Tau Pathology in Transgenic Alzheimer’s Mice Model
Ionic co-crystals of lithium salicylate with organic proline (LISPRO) showed better safety and pharmacokinetic profile of lithium in plasma and brain of wild-type and transgenic Alzheimer mice model compared to lithium salts.
Determination of the Fat Content Profile of different Chocolate Products using an Automated Workflow for the Generation of Fatty Acid Methyl Esters (FAME)
The method allows for automated determination of total fat content and quantitative analysis of saturated and unsaturated cis- and trans- fatty acids of different chocolate products.
Protein Precipitation without Pipetting
Salting out, adding a solvent, altering the pH are various means to precipitate proteins. In a subsequent two-step process, the mixture is then centrifuged and the supernatant is pipetted off to separate it from the precipitate.