A Novel Benchtop Time-of-Flight GC-MS System for High Performance Analysis of Human Urine
Poster Sep 13, 2016
David E. Alonso, Joseph E. Binkley, Lorne M. Fell
The development of improved profiling workflows are critical for effective investigation of environmental exposure and its relationship to chronic diseases. Metabolomic methods are particularly useful in this endeavor due to the proximity of metabolites to system phenotype, and their relatively quick insight into system perturbations from diet, drugs, gut microbiota, etc. (Figure 1). Unfortunately, a major problem with these methods continues to be incomplete sample characterization due to metabolite diversity, wide concentration range, and the overall complexity of biological matrices.
Urine is an ideal bio-fluid for studying the effects of exposure, since it is easy to obtain in large volumes, and is relatively free from interfering proteins and lipids. In addition, high concentrations of drugs and other xenobiotic materials can be detected over extended periods of time. In this study, we used a high performance, benchtop gas chromatography time-of-flight mass spectrometer for comprehensive characterization of derivatized urine samples (Figure 2).
Using Elemental Analysis For Discrimination Of Pinot Noir Wines From Six Different Districts In An AvaPoster
The determination of geographical origin of wine is gaining increased interest by researchers and federal agencies around the world, partially due to increased fraud with regards to place of origin labelling. For wine, multi-elemental profiling of macro, micro, and trace elements has been proposed for determination of authenticity. Commercial wines from different wineries in 5 different neighborhoods within one AVA show characteristic elemental fingerprints. Macro, micro and trace elements as well as elemental ratios contribute to the observed separation, indicating the involvement of multiple factors and underlying mechanisms, including location and soil composition, elemental uptake by vine and rootstock, viticulture and nutrient management, water sources, and small differences in the different wineries.READ MORE
Fast arsenic speciation analysis of wines and rice with LC-ICP-QQQPoster
This method was designed in response to recent and proposed food standards, both international and national, that limit inorganic arsenic rather than total, organic, or individual arsenic species such as arsenite (AsIII) and arsenate (AsV). Analysis time is 10x faster than the current FDA regulatory method, increasing sample throughput, avoided spectral interferences and dramatically increased sensitivity. Validation data from two laboratories demonstrate the method’s accuracy and reproducibility of both wine and rice matrices in a single analytical batch.READ MORE
Proteomics and Substrate Based MS Imaging of Xenobiotic Metabolising Enzymes in Ex Vivo Human Skin and a Human Living Skin Equivalent ModelPoster
Untargeted proteomics analysis showed that human skin and a commercially available living skin equivalent model exhibit a similar distribution of xenobiotic metabolising enzymes. A new technique, substrate based mass spectrometry imaging (SB-MSI) was developed during this study.READ MORE