A Novel Benchtop Time-of-Flight GC-MS System for High Performance Analysis of Human Urine
Poster Sep 13, 2016
David E. Alonso, Joseph E. Binkley, Lorne M. Fell
The development of improved profiling workflows are critical for effective investigation of environmental exposure and its relationship to chronic diseases. Metabolomic methods are particularly useful in this endeavor due to the proximity of metabolites to system phenotype, and their relatively quick insight into system perturbations from diet, drugs, gut microbiota, etc. (Figure 1). Unfortunately, a major problem with these methods continues to be incomplete sample characterization due to metabolite diversity, wide concentration range, and the overall complexity of biological matrices.
Urine is an ideal bio-fluid for studying the effects of exposure, since it is easy to obtain in large volumes, and is relatively free from interfering proteins and lipids. In addition, high concentrations of drugs and other xenobiotic materials can be detected over extended periods of time. In this study, we used a high performance, benchtop gas chromatography time-of-flight mass spectrometer for comprehensive characterization of derivatized urine samples (Figure 2).
Analysis of Urine SRMs Using Solid-Phase Micro Extraction, Dynamic Headspace, and Liquid Injection with Comprehensive GCxGC High Res TOFMSPoster
We used modern-day, high-resolution time-of-flight mass spectrometry (HRT) and powerful processing software to quickly and confidently identify compounds in urine.READ MORE
Screening Medical Marijuana for Pesticides by GC-MS/MSPoster
This poster describes streamlined method development process for analysis of pesticide residues in MM using a QuEChERS sample preparation method, followed by GC-MS/MS detection and quantitation.READ MORE
Determination of the Fat Content Profile of different Chocolate Products using an Automated Workflow for the Generation of Fatty Acid Methyl Esters (FAME)Poster
The method allows for automated determination of total fat content and quantitative analysis of saturated and unsaturated cis- and trans- fatty acids of different chocolate products.READ MORE
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14th Symposium on the Practical Applications of Mass Spectrometry in the Biotechnology Industry
Sep 19 - Sep 23, 2017