Digital microphotographic atlas of the adult zebrafish cerebellum

Sagittal, coronal and horizontal, one micron thick plastic (semithin) sections of adult zebrafish (Danio rerio) brain were cut serially, using an ultramicrotome, mounted on glass slides and stained with toluidine blue. Each cerebellar region of the brain sections was photographed in 40X using a computer assisted Olympus light microscope, montaged via Photoshop and arranged in series. Roughly 10 slices from each sectional orientation were selected. The resulting pictures are presented in pairs, so the three dimensional anatomical relationships of the zebrafish cerebellum could be visualized by preparing all three sections together. The final product resulted in a digital microphotographic atlas of the adult zebrafish cerebellum, which is a very useful tool to researchers alike.