Effective Fishing of Characteristic Proteome Fractions and Identification of Biomarkers Therein: Application of VisualCockpit to Multidimensional Chromatogram and MS Data
Poster Oct 11, 2006
S. Kreusch, M. Nagel, R. Janetzko, G. A. Cumme, A. Winter, M. Pohl, A. Meier-Hellmann and H. Rhode
Two different strategies may be used for fishing biomarkers via proteomics based methods :
1) All proteome fractions – eventually some thousands – generated from different samples are analyzed, quantified, and compared. This causes high operating effort and requires high throughput methods for analysis and data handling which are not available with all analytical procedures. This strategy provides the possibility to find all biomarker candidates detectable, however, their number is confined by the analytical methods at hand and the possible operating effort.
2) Otherwise, start with pre-selection of characteristic fractions and focus the operating expense on careful and comprehensive analysis of these fractions using an adequate assortment of different analytical strategies. With this strategy biomarker yield depends on the pre-selection criteria.
Depending on the separation procedure, characteristic fractions may be identified by double staining with 2DE, characteristic tags, other task-specific properties or simply by protein quantification.
Here we introduce an efficient pre-selection based on optical properties of the fractions. Logical selection criteria are applied to sequentially reduce the fractions that have to be analyzed further. Appropriate subsets of fractions are produced and visualized by the software package VisualCockpit. This software can be applied in principle to all data formats and to both fishing strategies, and to evaluation of separation procedures.
Inhibition of The Auto-inflammation Suppressor Protein ISG15 Triggers Preeclampsia by Blocking Trophoblast Migration and InvasionPoster
In summary, ISG15 expression levels are crucial for trophoblast morphology and function (migration/invasion). By blocking trophoblast invasion, reduced ISG15 levels could contribute to impaired spiral artery transformation that reduces utero-placental blood flow in preeclampsia. Thus, agents inducing ISG15 expression are likely to be therapeutic in preeclampsia.
An Emerging Phenotype of Partial RAG 1/2 Deficiency Among Young Children with Autoimmunity and Viral InfectionsPoster
We describe the natural history of a cohort of 12 patients with confirmed partial RAG1/2 mutations and autoimmunity at a young age. We were seeking the link between viral infections and autoimmunity and tested candidate biomarkers that may reflect the underlying RAG1/2 protein deficiency.READ MORE
Using Elemental Analysis For Discrimination Of Pinot Noir Wines From Six Different Districts In An AvaPoster
The determination of geographical origin of wine is gaining increased interest by researchers and federal agencies around the world, partially due to increased fraud with regards to place of origin labelling. For wine, multi-elemental profiling of macro, micro, and trace elements has been proposed for determination of authenticity. Commercial wines from different wineries in 5 different neighborhoods within one AVA show characteristic elemental fingerprints. Macro, micro and trace elements as well as elemental ratios contribute to the observed separation, indicating the involvement of multiple factors and underlying mechanisms, including location and soil composition, elemental uptake by vine and rootstock, viticulture and nutrient management, water sources, and small differences in the different wineries.READ MORE