Fast arsenic speciation analysis of wines and rice with LC-ICP-QQQ
Poster Dec 05, 2017
Patrick Gray, Courtney Tanabe, Susan E. Ebeler, Jenny Nelson.
This fit-for-purpose method was designed in response to recent and proposed food standards, both international and national, that limit inorganic arsenic rather than total, organic, or individual arsenic species such as arsenite (AsIII) and arsenate (AsV). In this method, AsIII is intentionally oxidized to AsV with H2O2 during sample preparation, converting all inorganic arsenic (the sum of AsIII and AsV) to the AsV form. Arsenic species were separated in less than 2 minutes using a short, narrow bore, 5 um chromatography column. This analysis time is 10x faster than the current FDA regulatory method. The use of O2 reaction gas with inductively coupled plasma triple quadrupole mass spectrometry (ICP-QQQ with MS/MS capability) avoided spectral interferences and dramatically increased sensitivity, allowing for low volume injections. The small injection volume and modified mobile phase composition mitigate non-spectral interferences such as carbon enhanced ionization. Furthermore, the shortened analysis time significantly increases sample throughput. Validation data from two laboratories demonstrate the method’s accuracy and reproducibility of both wine and rice matrices in a single analytical batch.
Results are presented that demonstrate the accuracy and reproducibility of the new method. The method was further validated using a wine matrix that was analyzed by two participating laboratories.
The method presented here has been shown to work for both wine and rice matrices. Runtime has been decreased to 2 minutes, which is a factor of 10x faster than previous methods. The method also showed improved sensitivity and limits of detection and quantification compared to the current FDA method. Small injection volumes and addition of methanol in the mobile phase increased robustness to non-spectral interferences and makes this method more suitable for wine speciation. If needed, analysis time and resolution could be further improved by decreasing injection volume, increasing mobile phase flow rate and using a small volume,fast-washout spray chamber.
Sport Doping Screening in Biological Matrices by Multi-Dimensional LC-QToFPoster
This work evaluated the performance of 2D LC variant using a QToF setup instead of a triple quadrupole mass spectrometer for the analysis of drug of abuse in urine targeting low and sub ppb level.READ MORE
Analysis of Doping and Forensic Drugs in Urine Using High-Resolution GC/Q-TOFPoster
In this study, we are examining the potential for high resolution accurate mass 7250 GC/Q-TOF equipped with low energy EI source, for both quantitative and screening aspects of doping control and forensic drugs applications.READ MORE
Automation of Sample Preparation for Metabolomic Analysis Using Robotic PlatformPoster
We developed an automated sample preparation protocol based on a robotic platform PAL RTC (CTC Analytics AG, Zwingen Switzerland), which represent a modified Bligh and Dyer method producing samples for both hydrophilic metabolomics using GC-MS and lipidomics using SFC-MS simultaneously.READ MORE