Herbal Metabolic Profiling of Raw and Steamed Panax notoginseng
Poster Dec 08, 2006
Eric C.Y. Chan, Swee-Lee Yap, Aik-Jiang Lau, Pay-Chin Leow, Ding-Fung Toh, and Hwee-Ling Koh
At present, metabolite profiling is of growing importance in herbal medicine fields such as breeding, formulation, quality control and clinical trials. Herbal metabolic profiling allows direct detection of down-stream derivatives of metabolites, arising from herbal formulation process, using metabolite profiling ultra performance liquid chromatography time-of-flight mass spectrometry (UPLC/TOFMS).
This analytical approach allows the discrimination and tentative authentication of unique biomarkers related to different herbal extracts using unsupervised multivariate principal component analysis (PCA) of the UPLC/TOFMS data. The tentative identification of biomarkers is complemented significantly by the accurate mass measurement of TOFMS and the high resolution and high retention time reproducibility rendered by UPLC.
The application of this approachin herbal extract discrimination and ginsenoside biomarker discovery of raw and steamed Panax notoginseng (Burk.) F.H. Chen is demonstrated and discussed.
Determination of the Fat Content Profile of different Chocolate Products using an Automated Workflow for the Generation of Fatty Acid Methyl Esters (FAME)Poster
The method allows for automated determination of total fat content and quantitative analysis of saturated and unsaturated cis- and trans- fatty acids of different chocolate products.READ MORE
Fast Pesticide Residue Analysis Using a Novel Benchtop Time-of-Flight Mass SpectrometerPoster
The objective of the study was to maximize throughput without introducing a significant number of additional chromatographic co-elutions not separable by deconvolution, whilst transitioning from a standard 30 m column to a 15 m column using LECO's software.READ MORE
Investigating the Effects of Fructose Consumption and Inadequate Copper Intake on Nonalcoholic Fatty Liver DiseasePoster
Metabolomics is a viable method for identifying compounds associated with NAFLD. In this investigation, high-quality data facilitate the identification of key metabolites differentiating normal versus diseased species.READ MORE