Revealing Host Metabolome Reprogramming by the Economically Important Fungal Plant Pathogens Botrytis cinerea and Magnaporthe grisea
Poster Mar 28, 2007
Aileen R., Smith J., William Allwood, Amanda J. Lloyd, David I. Ellis, Royston Goodacre and Luis A.J. Mur
Metabolomic approaches including Fourier- transform infrared (FT-IR) spectroscopy and Electrospray ionisation mass spectrometry (ESI-MS) were used to elucidate changes during susceptible and resistant interactions of Magnaporthe grisea with B. distachyon. Following data mining, discriminatory analytes (m/z) were shown to be phospholipids (PL) by ESIMS-MS. We are now moving to correlate transcriptional and metabolomic changes, concentrating on PL-based regulation.
This same Metabolomic approach is being applied to the Botrytis cinerea interaction with Arabidopsis wild-type and ethylene signalling mutants, which exhibit either enhanced or compromised defence.
Investigating the Effects of Fructose Consumption and Inadequate Copper Intake on Nonalcoholic Fatty Liver DiseasePoster
Metabolomics is a viable method for identifying compounds associated with NAFLD. In this investigation, high-quality data facilitate the identification of key metabolites differentiating normal versus diseased species.READ MORE
A Tissue-based Proteomic Study of VEGFR2 in Human Term PlacentasPoster
VEGFR2 is the main regulator of placental angiogenesis and at term is localized in endothelial cells (EC) of the villous vasculature. VEGFR2 immunoprecipitation (IP) of membrane proteins, extracted from the fetal compartment, isolated 30 proteins that were identified by proteomic analysis.READ MORE
RAMclust/RAMsearch: efficient post-XCMS feature clustering and annotation of MS-based metabolomics datasetsPoster
Chromatographically coupled mass spectrometry is a powerful tool for profiling, semi-quantitatively or quantitatively, a breadth of small molecules with sensitivity and selectivity.READ MORE