Sialylation and Metastasis: from tumor-associated antigens discovery to therapeutic development
Poster Jun 03, 2010
Aberrant sialylation affects the metastatic behaviour of cancer cells.We have quantified the sialylated cell surface glycoproteins from cells with different metastatic behaviour.The membrane proteins from isogenic human cell lines were analyzed by a method based on TiO2-HILIC-MS.1200 sialylated glycosites, 116 enzymes involved in the glycoconjugate metabolism and for the first time 4 glycosylation sites of Her2 receptor were identified, showing the importance of this tool for discovery and therapeutic intervention.
Application of Solid Phase Micro Extraction (SPME) and On‐Fiber Derivatization to Detect Precursors of Biomolecules in the study of Proto‐metabolismPoster
In the studies of origins of life, plausible synthetic reaction pathways to form biological relevant molecules, or building blocks of proto‐bilayer lipids, protein, RNA, and DNA in the primitive environment have been investigated intensively.READ MORE
A Tissue-based Proteomic Study of VEGFR2 in Human Term PlacentasPoster
VEGFR2 is the main regulator of placental angiogenesis and at term is localized in endothelial cells (EC) of the villous vasculature. VEGFR2 immunoprecipitation (IP) of membrane proteins, extracted from the fetal compartment, isolated 30 proteins that were identified by proteomic analysis.READ MORE