Specificity of small molecule inhibitors for deubiquitinating enzymes in living cells assessed by activity-based proteomics
Poster Aug 19, 2010
Small molecular compounds (PR-619 and P22077) was assessed for their abilities to inhibit DUB function in crude extracts and in cells. Activity-based profiling combined with quantitative mass spectrometry revealed the inhibitory capacity of a broad range of DUBs by the PR-619, whereas P22077 showed specificity towards subsets of DUBs including USP7 in cells. Our results demonstrate the usefulness of activity-based quantitative proteomics to monitor inhibition of endogenous DUBs in vivo.
A Tissue-based Proteomic Study of VEGFR2 in Human Term PlacentasPoster
VEGFR2 is the main regulator of placental angiogenesis and at term is localized in endothelial cells (EC) of the villous vasculature. VEGFR2 immunoprecipitation (IP) of membrane proteins, extracted from the fetal compartment, isolated 30 proteins that were identified by proteomic analysis.READ MORE
Automating Mass Spectrometry-Based Quantitative Glycomics using Tandem Mass Tag (TMT) Reagents with SimGlycanPoster
One of the emerging trends in glycomics research is the innovation related to accurate MS based quantitative analysis of glycans.READ MORE