TMT Quantification for Shotgun Proteomics: TIMS-QTOF Powered by PASEF
Poster May 31, 2019
Matt Willetts, Shourjo Ghose, Heiner Koch, Scarlet Koch, Markus Lubeck, Gary Kruppa.
This poster evaluates the performance of ‘timsTOF Pro’ a unique dual trapped ion mobility spectrometer (TIMS) coupled to a QTOF for the analysis of isobaric labelling experiments.
Download to discover:
- How TIMS provides an additional dimension of separation and high peak capacity
- Why the timsTOF Pro instrument is well suited to isobaric labelled quantitation experiments
- How to achieve near 100% duty cycle for shotgun proteomics
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.
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