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Anachem’s new DNAreleasy™ - Go from cells to PCR in 10 Minutes
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Anachem now has introduced new DNAreleasy™ to prepare sample for DNA amplification.
This new cell lysing reagent requires just 10 minute incubation on a thermal cycler before the DNA is ready for use directly in PCR – not further sample processing is necessary, the company says.
DNAreleasy™ has been successfully used to lyse a wide range of cells. This includes: human buffy coat, human fibroblasts tissue culture cells, human sperm, mouse & rat tissue culture cells, mouse tail, various plants, Drosophila and others like yeast, Gram +ve bacteria, Gram –ve bacteria, viruses and many more.
For using DNAreleasy™, mix cells with 20 µl of the reagent and overlay with mineral oil if necessary (for Thermal Cyclers without a heated lid). Place in a Thermal Cycler and treat at 75°C for 5 minutes followed by 96°C for 2 minutes before holding at 20°C. After the lysis, a part or all of the DNAreleasy-lysate can be used directly in PCR. DNAreleasy can make up to 40% of most PCR mixes. It can be stored at -20°C for future use.
This new cell lysing reagent requires just 10 minute incubation on a thermal cycler before the DNA is ready for use directly in PCR – not further sample processing is necessary, the company says.
DNAreleasy™ has been successfully used to lyse a wide range of cells. This includes: human buffy coat, human fibroblasts tissue culture cells, human sperm, mouse & rat tissue culture cells, mouse tail, various plants, Drosophila and others like yeast, Gram +ve bacteria, Gram –ve bacteria, viruses and many more.
For using DNAreleasy™, mix cells with 20 µl of the reagent and overlay with mineral oil if necessary (for Thermal Cyclers without a heated lid). Place in a Thermal Cycler and treat at 75°C for 5 minutes followed by 96°C for 2 minutes before holding at 20°C. After the lysis, a part or all of the DNAreleasy-lysate can be used directly in PCR. DNAreleasy can make up to 40% of most PCR mixes. It can be stored at -20°C for future use.
