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AnaSpec Introduces SensoLyte 520 Calpain Kit
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AnaSpec has announced the release of the SensoLyte 520 Calpain Activity Assay Kit, the industry’s first longest wavelength FRET based calpain assay. Optimized for detecting calpain activity, this kit contains a novel internally quenched 5-FAM/QXL™ 520 FRET substrate.
Cleavage of this FRET substrate generates two separate fragments. The subsequent release of 5-FAM fluorescence can be monitored at excitation/emission= 490/520 nm. Increase in fluorescence is proportional to the calpain activity. The assay can detect both calpain 1 (m) and 2 (m) activities and is ideal for kinetic study of these enzymes. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds.
An AMC based calpain assay, the SensoLyte® AMC Calpain Assay, is also available from AnaSpec.
Cleavage of this FRET substrate generates two separate fragments. The subsequent release of 5-FAM fluorescence can be monitored at excitation/emission= 490/520 nm. Increase in fluorescence is proportional to the calpain activity. The assay can detect both calpain 1 (m) and 2 (m) activities and is ideal for kinetic study of these enzymes. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds.
An AMC based calpain assay, the SensoLyte® AMC Calpain Assay, is also available from AnaSpec.
