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AnaSpec Introduces Ultra-Sensitive HCV NS3/4A Protease FRET Substrate

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AnaSpec has developed a tool for optimized detection performance, focusing on the NS3/4A protease.

NS3/4A protease is the protease responsible for the cleavage of HCV nonstructural polyprotein at the NS3-NS4A, NS4A-NS4B, NS4B-NS5A, and NS5A-NS5B sites.

Since these cleavages are essential for the maturation of the viral proteins, this protease has become one of the key targets for developing anti-HCV drugs. Applying both peptide and fluorescent dye technologies, AnaSpec has developed an ultra-sensitive HCV NS3/4A protease FRET substrate that is designed to detect the presence of HCV NS3/4A protease at levels of < 0.1 pmol. 

Based on the peptide sequence, Ac-DED(EDANS)EE-Abu-[y,COO]ASK(DABCYL)-NH2, developed by Taliani, M. et al. (1), the donor and the quencher have been substituted with 5-FAM and our trademark quencher QXLTM 520, respectively.

This substrate, Ac-DE-DAP(QXL520)-EE-Abu-[y,COO]AS-C(5-FAMsp)-NH2, shows a 22-fold lower Km than the EDANS/DABCYL peptide.