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BIOCRATES Announces Launch of its Second MS-Based Metabolomics Research Kit - AbsoluteIDQ p180 Kit

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BIOCRATES announces the launch of the latest addition to its products and services portfolio, the AbsoluteIDQ p180 Kit. The new kit, an extended version of the first-ever mass-spectrometry–based metabolomics kit developed by BIOCRATES, quantifies 186 metabolites from 5 compound classes, i.e., acylcarnitines, amino acids, phospho- and sphingolipids, hexoses, and biogenic amines.

The AbsoluteIDQ p150 Kit is designed for use with AB Sciex API 4000™ or 4000 QTRAP® mass spectrometers, it enables researchers to measure all of their sensitive sample material and to analyze their proprietary data right in their own lab.

The BIOCRATES AbsoluteIDQ p180 Kit is a combined FIA-MS/MS and LC-MS/MS assay for sensitive quantification. Quantification utilizes stable isotope-labeled or chemically homologous internal standards. The patented plate design ensures efficient sample derivatization and reproducibility in analyte extraction. The assay is suitable for both manual and automated high-throughput operation and works with sample volumes as small as 10 µL.

The 5 compound classes analyzed by AbsoluteIDQ p180 Kit cover a wide range of relevant physiological events, including energy metabolism, fatty acid transport and mitochondrial fatty acid oxidation, ketosis, oxidative stress, or mitochondrial membrane damage (acylcarnitines), amino acid metabolism, urea cycle, glycolytic or gluconeogenetic activity, insulin sensitivity or resistance, or neurotransmitter metabolism (amino acids), carbohydrate metabolism (hexoses), dyslipidemias, membrane composition and damage, fatty acid profile, activity of desaturases, signaling cascades, or membrane damage (sphingomyelins) as well as neurological disorders, cell proliferation, or cell cycle progression (biogenic amines).

The MetIQ Software that ships with the kit streamlines data analysis, calculating metabolite concentrations in an automated manner and offering a number of output formats.

Being based on targeted metabolomics, the AbsoluteIDQ p180 Kit not only eliminates the need for metabolite identification, it provides quantitative data that are annotated. This assay is a choice for researchers who are looking for a solution to measure metabolite panels under quality-controlled conditions in their own laboratory, even at high throughput.