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Bio-Rad Introduces Protein Expression, Purification, and Tag Removal System Fast Forward to Tag-Free Protein

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Bio-Rad Laboratories, Inc. has announced the new Bio-Rad Profinity eXact™ Fusion-tag System, an integrated set of products that address bottlenecks in the tag-removal process involved with the purification of affinity-tagged proteins.

The result of the entire process is true, single-step purification without the difficulty and expense of cleavage enzymes, incubation times, or removal of reagents.

The Profinity eXact fusion-tag system utilizes an immobilized, engineered Subtilisin protease to carry out affinity binding and tag cleavage, which shortens the purification process.

As the ligand, the protease is not only involved with the binding and recognition of the tag, but upon application of the elution buffer, it also serves to cleave the tag from the fusion protein directly after the cleavage recognition sequence. This delivers a native, tag-free protein in a single step.

“Profinity eXact enables researchers to purify tag-free proteins in one hour,” said Tanis Correa, senior product manager of Bio-Rad Lab Chromatography products. “It’s an excellent option for scientists utilizing the simplicity of affinity purification, but whose applications demand unadulterated protein for functional and structural analyses.”

The key benefits of the Profinity eXact system include purification and processing of fusion-tagged proteins in a single step, on-column cleavage in as little as 30 minutes, elimination of a protease addition step, and precise cleavage at the N-terminus to generate a native protein sequence.