Food Allergen Screening Method by SCIEX Receives Official Method Classification from the AOAC INTERNATIONAL
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SCIEX, a global leader in life science analytical technologies, announced that its highly sensitive method for analyzing food allergens has received the First Action Official Method (FAOM) classification from AOAC INTERNATIONAL’s Official Methods Board (OMB). Nearly 150 million people worldwide suffer from a food allergy and are allergic to more than one food. Accurate food allergen tests are urgently needed, to help reduce the risk of food labeling errors. It is therefore vital that the industry adopts improved food testing methods, and the SCIEX method is the first mass spectrometry (MS)-based method to receive FAOM classification for allergen screening.
The use of MS technology instead of more traditional methods such as polymerase chain reaction (PCR) or immunoassay-based approaches gives far greater confidence for detecting allergens in complex food samples. The SCIEX method for Allergen Screening uses liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) to analyze a range of food products (both raw and baked goods) by detecting several unique signature peptides that are specific to each allergen. Presently, this method can detect allergenic peptides from five of the major classes of allergenic foods at a detection limit of 10 ppm in a variety of food matrices. Plus, with increased throughput, the ability to identify multiple key allergens at once from a number of different food types, and advanced peptide signature detection, it greatly reduces the chance of false negative and false positive results.
“Traditional methods such as ELISA and PCR for allergen analysis come with several challenges, including detection accuracy in processed products or the costliness of testing for several allergens in the same sample,” said Dr. Bert Pöpping, FOCOS Consulting. “LC-MS/MS has the potential to overcome these challenges, which is just one reason why it is being adopted by multiple governments and private laboratories for allergen detection.”
The method uses the power and robustness of the QTRAP® 4500 LC-MS/MS System for high throughput trace screening and quantitation of allergens. LC-MS/MS enables comprehensive sample data analysis, making food allergen analysis more accurate and, ultimately, safer for consumers.
The use of MS technology instead of more traditional methods such as polymerase chain reaction (PCR) or immunoassay-based approaches gives far greater confidence for detecting allergens in complex food samples. The SCIEX method for Allergen Screening uses liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) to analyze a range of food products (both raw and baked goods) by detecting several unique signature peptides that are specific to each allergen. Presently, this method can detect allergenic peptides from five of the major classes of allergenic foods at a detection limit of 10 ppm in a variety of food matrices. Plus, with increased throughput, the ability to identify multiple key allergens at once from a number of different food types, and advanced peptide signature detection, it greatly reduces the chance of false negative and false positive results.
“Traditional methods such as ELISA and PCR for allergen analysis come with several challenges, including detection accuracy in processed products or the costliness of testing for several allergens in the same sample,” said Dr. Bert Pöpping, FOCOS Consulting. “LC-MS/MS has the potential to overcome these challenges, which is just one reason why it is being adopted by multiple governments and private laboratories for allergen detection.”
The method uses the power and robustness of the QTRAP® 4500 LC-MS/MS System for high throughput trace screening and quantitation of allergens. LC-MS/MS enables comprehensive sample data analysis, making food allergen analysis more accurate and, ultimately, safer for consumers.
