We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.


Introducing the new Diffusion Measurement Package for the Olympus FluoView FV1000 Confocal Microscope

Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "Introducing the new Diffusion Measurement Package for the Olympus FluoView FV1000 Confocal Microscope"

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

Olympus has introduced the new Diffusion Measurement module for Olympus ASW 2.1 software. Designed specifically for use with the Olympus FluoView FV1000 confocal microscope system for live cell imaging, this new software module provides users with the flexibility to undertake three different types of diffusion study: point fluorescence correlation spectroscopy; raster scan image correlation spectroscopy (RICS) and fluorescence recovery after photobleaching (FRAP). As a result, the Diffusion Measurement Package is ideal for a broad range of cell biology and biophysics applications.

Intracellular diffusion is frequently analyzed to assess the movements, interactions and microenvironments of intracellular molecules to determine the patterns and rates of molecular movement. In order to meet this need, the Olympus Diffusion Measurement Package enables researchers to execute three different types of diffusion study.

Point Fluorescence Correlation Spectroscopy

Data is obtained from a single location using point scanning, enabling fluctuations in fluorescence intensity to be recorded. The number of moving particles within the confocal volume is therefore easily calculated. With its high temporal and spatial resolution, this technique provides data on the diffusion constant or number of molecules, as well as the capability to analyze cross correlations between different molecules.

With the ability to provide diffusion measurements in various cellular regions, the software is able to present the user with a spatial mapping of diffusion time constants. As a result, users can easily define small regions of interest and subsequently create a diffusion map from a series of 2D images. A wide range of intracellular structures, from molecules in solution to cell membrane proteins, can therefore be accurately measured. Resulting data can be expressed as either a diffusion constant or the number of available molecules.

As a widely used fluorescence analysis technique, researchers commonly use FRAP to analyze the diffusion constant in a particular region of the cell, as molecules diffuse into the area after photobleaching.

The Diffusion Measurement Package enables users of the Olympus FluoView FV1000 to control and measure the diffusion constant with ease.