Sigma-Aldrich Launches PhosphoProfile™ I Phosphopeptide Enrichment kit

Sigma-Aldrich Research Biotech, a division of Sigma-Aldrich, has launched the PhosphoProfile™ I Phosphopeptide Enrichment Kit, a kit for the enrichment of phosphopeptides.

"This launch of the new phosphopeptide enrichment kit demonstrates Sigma-Aldrich's commitment to develop and offer cutting-edge proteomic technologies," said George Lipscomb, Product Manager, Proteomics, for Sigma-Aldrich's Research Biotech division.

"We are proud to further enable the discovery and success of researchers through innovative technologies and service."

The technology is based on a Ga+3 chelate on silica that provides a method for phosphoproteomics.

This approach is designed to offer several advantages to proteomics researchers, including low non-specific binding, no bias in selectivity of phosphorylation state, high sensitivity, and sample handling, especially for small samples.

Phosphoproteins are digested with the included Proteomics Grade Trypsin, resulting in high-quality phosphopeptides that are captured in the convenient spin-tubes.

Recovered phosphopeptides are then ready for analysis by either MALDI-TOF or ESI mass spectrometry.

Up to 25 nanomoles of phosphopeptide can be captured and recovered from complex samples.

Also included are the necessary buffers and mono and tetra phosphopeptide controls.