Thermo Fisher Scientific Releases SurePac Bio 550 SEC MDi and DNAPac RP Semi-Preparative Columns at HPLC 2024

To support today’s modern laboratories, Thermo Fisher Scientific is introducing several new columns to meet researchers’ analytical, efficiency, and scalability needs at HPLC 2024, taking place in Denver, CO from July 20 – 25. The Company is releasing new Thermo Scientific™ SurePac™ Bio 550 SEC MDi™ columns to enable fast separations of adeno-associated virus monomers and aggregates. Additionally, the Thermo Scientific DNAPac™ RP HPLC columns are now available in semi-preparative formats to offer scalability for researchers developing and producing DNA/RNA oligonucleotides.  

The Thermo Scientific SurePac Bio 550 SEC MDi columns feature 3 µm monodisperse particles and inert (MDi) column hardwares to provide superior adeno-associated viruses (AAV) monomer retention and higher resolution of aggregates in cell and gene therapy applications. The columns enable fast run times and help achieve optimal separation efficiency and reliable results to accelerate the delivery of novel therapeutics to market.  

The Thermo Scientific DNAPac RP semi-preparative columns are designed to help laboratories navigate the expanding field of oligonucleotide therapies and precision medicine. The unique chemistry of the DNAPac RP semi-preparative column delivers unmatched performance across wide ranges of pH, temperature, and mobile phase compositions for precise separations and sample purification.  

Key Applications: 

Thermo Scientific SurePac Bio 550 SEC MDi analytical columns and guards 

• Gene-therapy and biopharmaceutical analytical laboratories that require size exclusion chromatography (SEC) for determination of aggregates in AAV analysis.  

Thermo Scientific DNAPac RP semi-preparative column 

• Laboratories working with synthetic oligonucleotides, siRNA, ASO, and mRNA to achieve lab-scale purification of small and large DNA and RNA oligonucleotides.   

Features/Benefits: 

Thermo Scientific SurePac Bio 550 SEC MDi column 

• Enhanced efficiency and resolution: high-resolution separation of AAV and aggregates through the power of monodisperse particles to improve the homogeneity of packing and reduce peak broadening effects such as eddy-diffusion 
• Reproducibility: MDi technology ensures consistent particle size distribution for column-to-column and batch-to-batch reproducibility to achieve consistent results and robust, easy-to-validate methods.  
• Higher throughput: the column’s 3 µm particles enables the use of shorter columns to achieve the desired resolution compared to traditional 5 µm technologies unlocking significant throughput improvements with faster run times and ability to process more samples. 

Thermo Scientific DNAPac RP semi-preparative column 

• High-performance and resolution: achieves superior reversed-phase resolution and efficient separation in lab-scale purifications 
• Compatibility and versatile analysis: The particles’ wide operation pH range (0-14) and high-temperature range (up to 110 °C) makes it ideal to further support method development.  
• Flexible distribution: column chemistry based on a super-macroporous structure with a 4 µm DVB particles and pore size range of up 2000 Å, offering excellent separation of both short and long single- and double-stranded nucleic acids, including RNA modalities such as intact mRNA.