Thermo Scientific Introduces Kit for Protein Preparation from Five Subcellular Compartments
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Thermo Fisher Scientific has introduced the Thermo Scientific Subcellular Protein Fractionation Kit for Tissue. The Subcellular Fractionation Kit enables the step-wise preparation of cytoplasmic, membrane, soluble nuclear, chromatin-bound and cytoskeletal protein fractions with less than 15 percent cross-contamination in two hours.
The resultant samples are ideal for downstream analyses, including Western blotting, protein quantitation, electrophoretic mobility shift and reporter gene assays.
The versatile kit is optimized for use with a variety of tissue types, including liver, heart, brain, kidney, lung and spleen.
The Subcellular Protein Fractionation Kit contains optimized formulations and protocols for the specific protein fractionation of tissues samples, saving researchers time and money.
The kit also includes the uniquely designed Thermo Scientific Pierce Tissue Strainer, which quickly separates cells or lysates from tissue debris after mechanical disruption.
The device contains a 250-micron mesh filter and fits into standard 15-milliliter conical tubes. The Pierce® Tissue Strainer is also available separately.