ELISA – Multimedia

The host systems often used to manufacture protein-based biotherapeutics can produce their own endogenous proteins, known as host cell proteins (HCPs), which can ultimately contaminate the product. Download this list to learn about some of the most commonly used methods for HCP analysis.

CaptSure DIY ELISA is the latest cutting-edge ELISA kit with a built-in system for antibody labeling, enabling the rapid and easy development of in-house ELISAs using any antibody pair while eliminating the need to perform plate coating procedures.

Spinal muscular atrophy (SMA) is an inheritable cause of infant mortality that is characterized by the loss of lower motor neurons and skeletal muscle atrophy. The degeneration of motor neurons is caused by insufficient levels of survival motor neuron (SMN) protein, which is encoded by two nearly identical genes SMN1 and SMN2. Most cases of SMA harbour homozygous deletions of the SMN1 gene and retain at least one copy of SMN2.

There is no one-size-fits-all method for validating antibodies. This list details validation approaches that are common to all applications, and highlights things to consider for specific applications, such as detecting post-translational modifications.

This poster will show you how PASEF scans can be applied to HCP analysis, how it can improve sensitivity in peptide mapping whilst also providing high confidence in protein ID.

Introducing the latest DIBE technology for reliable HCP detection in biologics production. This brochure will help you learn about the challenges of current coverage assays, the advantages of streamlining, and the benefits of synchronized solutions for 2D-DIBE analysis.

Standard ELISA techniques for detecting protein biomarkers in serum or other biological samples can leave you with sensitivity that doesn’t quite cut it, analyte cross-reactivity, and reproducibility that’s ok at best.

This study found that coffee solutions treated at 5% are toxic to neurons. However, the toxicity reduced significantly at 2.5%. While the toxicity was significantly less allowing for more cells to survive at 2.5%, the levels of toxic amyloid beta 1-42 significantly reduced. This reduction in amyloid beta is associated with improvement in cognitive performance, as the presence of these toxic peptides is one of the characteristics of AD pathophysiology.

Favored for its simplicity, specificity and cost-effectiveness, ELISA is a useful technique used in many laboratories globally.

We describe the natural history of a cohort of 12 patients with confirmed partial RAG1/2 mutations and autoimmunity at a young age. We were seeking the link between viral infections and autoimmunity and tested candidate biomarkers that may reflect the underlying RAG1/2 protein deficiency.