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ELISA – Multimedia

New LEGENDplex™ Multi-Analyte Flow Assay Panels for Simultaneous Quantification of 13 Th Cytokines in Human and Mouse Samples content piece image
Poster

New LEGENDplex™ Multi-Analyte Flow Assay Panels for Simultaneous Quantification of 13 Th Cytokines in Human and Mouse Samples

We have developed two multiplexed assay panels, human and mouse, using fluorescence–encoded beads, suitable for use on various flow cytometers. Both panels allow simultaneous quantification of 13 human or mouse cytokines, including interleukins (IL-2, 4, 5, 6, 9, 10, 13, 17A, 17F, 21, 22), IFN-? and TNF-a. These Th cytokine panels can be used for serum, plasma, cell culture supernatant and other sample types, offering useful tools for biomedical research and drug discovery.
A multianalyte algorithm PCR-based blood test outperforms single analyte ELISA-based blood tests for neuroendocrine tumor detection content piece image
Poster

A multianalyte algorithm PCR-based blood test outperforms single analyte ELISA-based blood tests for neuroendocrine tumor detection

In a prospective study, in age-/sex- and ethnicity-matched patients and controls (n=82), a 51 panel multigene blood transcript test (NETest) was identified to be significantly more sensitive and accurate (>93%) than any single analyte assay (Chromogranin A, Pancreastatin or Neurokinin A) for neuroendocrine tumor detection.
App Note / Case Study

Efficient washing of magnetic beads

The use of magnetic beads as the solid phase in a multiplexed ELISA enables high throughput cytokine screening and offers several advantages over common ELISA assays with direct coupling of the analyte to the well-surface.
App Note / Case Study

Automating ELISAs on Tecan’s Freedom EVO® using Optimiser™ technology from Siloam Biosciences

One of the challenges in life sciences research today is to discover methods for running key assays more quickly, more reliably and using lower volumes of reagents and sample, but still with improved sensitivity. One area where this particularly holds true is for traditional enzyme-linked immunosorbent assays (ELISAs), whose application provides a useful measurement of antigens, including cytokines and a host of other biomarkers.
App Note / Case Study

Can LC-MS/MS Be Used in Horse Meat Detection?

Following the Food Standards Agency’s announcement that horse and pig DNA had been identified in beef products sold by several supermarket chains, further testing has revealed widespread incidences of such contamination. This application note details a rapid, robust, sensitive and specific LC-MS/MS assay for the simultaneous detection of horse meat and the banned substance phenylbutazone (BUTE).
App Note / Case Study

Automation of a Homogeneous Proximity Assay for Immunogenicity Testing of Biological Drug Products

Several challenges have surfaced during clinical evaluation of biological drug products due to a commonly associated immune response in patients. Anti-drug antibodies (ADA) are known to be frequently generated during administration of humanized monoclonal antibody therapeutics. A commonly used technology platform for assessment of immunogenicity relies on the bridging immunogenicity assay format typical of the ELISA.
Laser Welded Microfluidic Plasma Seperation Unit with On-Chip Pressure Conversion content piece image
Poster

Laser Welded Microfluidic Plasma Seperation Unit with On-Chip Pressure Conversion

This poster shows how microfluidic components for the separation of plasma from full blood can be cost-effective, with reliable processing and the production of quality plasma.
Automation of a Novel Cell-Based ELISA for Cell Signaling Pathway Analysis content piece image
Poster

Automation of a Novel Cell-Based ELISA for Cell Signaling Pathway Analysis

Monitoring and quantifying cell signaling pathways is critical for understanding the behavior of cell processes and many disease states. Monitoring and quantifying cell signaling pathways is critical for understanding the behavior of cell processes and many disease states.
Modular Bench Top Automation content piece image
Poster

Modular Bench Top Automation

ELISA is one the most utilized assay formats in biomedical research. Numerous clinical, veterinary, and research assays use the specificity of antibodies to identify a diverse array of analytes from any number of different matrices. This poster details a modular bench top workstation capable of automating the assay process steps of most conventional ELISA assays.
Novel Serological Assay Using HIgh-Throughput Chromogenic Microarray Technology for Screening SPF Non-human Primates content piece image
Poster

Novel Serological Assay Using HIgh-Throughput Chromogenic Microarray Technology for Screening SPF Non-human Primates

Routine surveillance programs to detect microbial agents in non-human primate colonies are critical to maintaining colony health and safety, as well as research integrity. Serological testing is a crucial element of maintaining colony health, but current methods rely on slow, expensive, and low-throughput ELISA-based serological assays. Here we describe the development of a sensitive, specific, robust, and easily automated method for colony health screening programs.
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