Immunoassays – Multimedia

The University of Kansas in collaboration with PerkinElmer Inc. worked on looking at the comparison of the AlphaLISA Technology and an Electrochemiluminescence Technology to measure assay windows, lower and upper detection limits and intra - and inter-assay precision. In this study, three AlphaLISA no-wash assays, which employ a faster and less complex protocol, were found to deliver highly sensitive and accurate results, equivalent to those obtained in ECL technology.

SiRNA designed to target the human VEGF gene promoter revealed a putative candidate for transcriptional gene silencing. However, mutation or deletion of the target site demonstrated that the observed VEGF knockdown was the result of a sequence-specific off-target effect. Bioinformatic and microarray analyses of genes implicated in VEGF transcriptional control followed by knockdown and Western blotting experiments presented one candidate, GRB2, as an unintended target of the VEGF promoter-specifi

Transcreener™ ADP2 Assay kits are far-red competitive fluorescence polarization immunoassays based on the detection of ADP. Any enzymatic reaction that uses ATP in a range from 0.1-1000 µM can be monitored. With its dual emission detection and five photomultiplier tubes, BMG LABTECH´s PHERAstar Plus provides the speed and sensitivity needed to take full advantage of BellBrook Labs Transcreener™ technology. Furthermore, a specific optical module was developed, thereby making assay setup simple.

Prostanoids originate from PGHS action on the 20-carbon polyunsaturated fatty acid: DGLA, AA and EPA. We used cDNA expression in Saccharomyces cerevisiae and enzyme immunoassays to compare the activities of two mouse PGHS isoforms and cDNAs encoding mouse prostaglandin I synthase and thromboxane A synthase and genomic DNA encoding Trypanosoma brucei prostaglandin F synthase. All genes were active and were put in Transgenic Arabidopsis thaliana plants producing DGLA.

Our aim is to develop a dedicated tool for multi-analyte detection in food based on IBIS Surface Plasmon Resonance imaging platform using a microarray format. Utilizing a multi-immunoassay on top of the SPR- imaging platform will allow rapid and simultaneous detection of various food ingredients and contaminants (e.g. food allergens, residues of veterinary drugs and environmental contaminants), providing the end user with a detailed food profile.

Transcreener™ KINASE ASSAY kits are far-red competitive fluorescence polarization immunoassays based on the detection of ADP. Any enzymatic reaction that uses ATP in a range from 1-250 µM can be monitored. With its dual emission detection and five photomultiplier tubes, BMG LABTECH´s PHERAstar provides the speed and sensitivity needed to take full advantage of BellBrook Labs Transcreener™ technology. Furthermore, a specific optical module was developed, thereby making assay setup simple.

To facilitate the rapid identification of PDE inhibitors, BellBrook Labs has developed a fluorescence polarization immunoassay that directly detects AMP and GMP. The Transcreener™ PDE Assay includes a single set of reagents - an anti-AMP/GMP antibody and fluorescent tracer - that can be used for any cyclic nucleotide PDE, providing a robust, cost-effective alternative to currently available technologies.

To facilitate the rapid identification of PDE inhibitors, BellBrook Labs has developed a fluorescence polarization immunoassay that directly detects AMP and GMP. The Transcreener™ PDE Assay includes a single set of reagents - an anti-AMP/GMP antibody and fluorescent tracer - that can be used for any cyclic nucleotide PDE, providing a robust, cost-effective alternative to currently available technologies.

We have developed a microarray based immunoassay to permit both greater food panel diversity and higher throughput testing. The Genarrayt™ 200+ Foods IgG test comprises of glass slides onto which 16 microarrays of over 200 different foods have been printed. Each microarray includes standards for quantitation and positive and negative controls for quality control. Food IgGs are detected by a novel fluorescent dye labelled anti-human IgG conjugate and results are measured using a laser scanner.

The LacZ reporter fusion assay is an excellent method to precisely quantify knockdown efficiency of siRNA-sequences and can be corroborated by Western blot analysis. Efficient production of Lentiviruses and high transduction efficiency (up to 98%) were achieved on lymphoid B- and T-cells as demonstrated by FACS analysis and GFP expression. Successful knockdown effect with specific shRNA was observed in suspension cells three days after lentiviral infection.