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Measuring Metabolic Flux with Cellular and Molecular Resolution: Combining Seahorse XF and LC/MS Technologies for Comprehensive Cellular Analyses

Speaking in June 2021 at the online symposium, Advances in Proteomics & Metabolomics, Dr. Genevieve Van de Bittner, R&D Researcher at Agilent Research Laboratories presented her talk on metabolic rate measurements using Seahorse XF and LC/MS technologies.

Abstract:  Alterations in metabolic flux have been linked to a wide variety of cell processes, from cancer development to T cell activation during immunotherapy or infection. This talk will cover two complementary technologies for measuring metabolic flux, Seahorse XF analyzers and liquid chromatography-mass spectrometry (LC/MS) qualitative flux analysis. Both technologies provide important insights into the rates of metabolic reactions within cells, with Seahorse XF measurements providing cellular resolution and LC/MS qualitative flux measurements providing molecular resolution. This presentation will highlight vignettes of combined Seahorse XF and LC/MS qualitative flux analyses that provide a comprehensive view of metabolic shifts in adipocytes during cool-temperature adaptation as well as various metabolic flux dependencies found across different cancer cell lines, including methods for targeting these metabolic dependencies.

We hope in this session you will:

  • Gain familiarity with metabolic rate measurements accessible to users of Seahorse XF and LC/MS technologies.
  • Understand biological insights that can be obtained through the combination of Seahorse XF and LC/MS measurements.

For Research Use Only. Not for use in diagnostic procedures.

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