Targeted Proteomics: From Discovery Plasma Proteomics to Clinically Actionable Protein Measurements

Discovery plasma proteomics is generating valuable biological insights, but translating relative protein signals into clinically actionable measurements remains a challenge. Variability across cohorts, laboratories, and platforms can limit reproducibility and data comparability.

In this episode of Teach Me in 10, Dr. Fredrik Edfors, assistant professor in systems biology at KTH Royal Institute of Technology, explains how targeted LC-MS/MS assays measure selected proteotypic peptides as proxies for proteins, and how stable isotope-labelled standards transform variable signals into robust quantitative ratios. 

Watch this episode to explore:

• How targeted LC-MS/MS enables more reliable protein quantification
• Why stable isotope-labelled standards improve reproducibility across studies
• Why protein-level standards, multi-peptide quantification, and standardized workflows are essential for clinical interpretability

• Edfors Lab
• The Human Protein Atlas
• About Quantitative Recombinant Protein Standards