Exploiting the timsTOF and MALDI-2 Platforms to Assess Pharmacodynamics and Pharmacokinetics in Preclinical Models
An integrated evaluation of the pharmacodynamic and pharmacokinetic properties of a drug is essential for successful translation to the clinic. However, cost-effective methods to measure these parameters at the systems level in pre-clinical models are lacking. Here, we introduce a timsTOF and MALDI-2 workflow to evaluate therapeutic activity. The platform combines ion mobility, mass spectrometry-based imaging, absolute drug quantitation across different biological matrices, in vivo isotope tracing, and global metabolome analysis in the adult zebrafish.
Effective Nuclei Isolation From Tissue for Single-Nucleus Sequencing
In this webinar, we present a standardized workflow solution, including easy and automated nuclei extraction and isolation for reproducible single-nucleus sequencing analysis.
Discovering Reprogrammed Molecular Pathways in Cancer Metastasis With MALDI Imaging
This presentation will highlight our recent progress in the discovery of N-glycans and phospholipids that significantly contribute to aberrant cancer metabolic pathways in primary tumors and metastases. We have also developed novel MALDI imaging approaches of drugs and drug metabolites that exploit key metabolic differences in tumors.
Scaling Up Deep Spatial Phenotyping of the Tissue Microenvironment
Join us for an invigorating spatial discussion, where we show the latest data using the PhenoCycler™-Fusion system, the fastest single-cell spatial biology solution.
Assessment of Biotherapeutics Quality Attributes Leveraging a Novel Fragmentation Technique
The novel fragmentation technique, electron activated dissociation (EAD) of the ZenoTOF 7600 system (SCIEX), was employed for peptide mapping of a multispecific monoclonal antibody (mAb) as well as IgG1 and IgG4 mAb samples. A specific focus was put on assessing challenging PTMs, such as glycosylations, amino acid isomers and the localization of a sulfation on a singly charged peptide.
Developing Mass Spectrometry for Deep and Quantitative Single-Cell Proteomics
Dr. Nemes will share developments from his lab that enabled the in situ and in vivo measurement of metabolites and proteins in single cells in live tissues and organisms, while Dr. Orsburn will explain how leaps in mass spectrometry can facilitate unprecedented sequence coverage of protein at the single-cell level.